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Standard Operating Procedures (SOPs) provide a detailed description of commonly used procedures.

Note that the use of the words ‘should’ and ‘must’ in these SOPs is consistent with their defined use in Canadian Council on Animal Care (CCAC) guidelines. The term 'should' indicates an obligation, for which any exceptions must be justified to, and approved by, an Animal Care Committee. The term 'must' indicates a mandatory requirement.

Any deviation from the approved procedures must be clearly described and justified in the Animal Use Protocol application in AMS. Approval of the protocol indicates approval of the deviation from the SOP for that project only.

The following Standard Operating Procedures (SOPs) were created and/or revised by the Veterinary Committee and approved by the Animal Policy and Welfare Oversight Committee (APWOC).

If you have questions or have a recommendation to make, please send an animalcare [at] mcgill.ca (email) or talk to the Veterinarian in your area.

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SOPs

Controlled Substances

SOP 401 on Cannabis and Controlled Substances.

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SOP 101-Rodent Analgesia	Ìý
SOP 103-NHP Analgesia	Ìý
SOP 106-Swine Analgesia	Ìý
SOP 107-Bird Analgesia	Ìý
SOP 108-Fish Aquatic Amphibian Reptile Analgesia	Ìý

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ÌýSOP 110 - Mouse Anesthesia	SOP 119 - Swine Anesthesia
ÌýSOP 111 - Rat Anesthesia	SOP 120 - Rodent Anesthesia Protocol Lung Physiology Measurements
ÌýSOP 115 - NHP Anesthesia	SOP 121 - Bird Anesthesia
SOP 118 - Fish and Aquatic Amphibian Anesthesia	Ìý

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ÌýSOP 201 - Rodent Surgery	SOP 205 - Rodent Tumor Resection
ÌýSOP 202 - Rodent Stereotaxic Surgery	SOP 206 - Rodent Ovariectomy
ÌýSOP 203 - Large Animal Surgery	SOP 207 - Rodent Castration
ÌýSOP 204 - Xenopus Egg and Oocyte Collection	SOP 208 - Rodent Mammary Fat Pad Injection

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ÌýSOP 301 - Rodent Euthanasia	ÌýSOP 304 - Avian Euthanasia
ÌýSOP 302 - Large Animal Euthanasia	ÌýSOP 305 - Transcardiac Perfusion
ÌýSOP 303 - Fish and Aquatic Amphibian Euthanasia	Ìý

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Ìýº£½ÇÉçÇø's EHS SOP 401 - Controlled Substances	ÌýSOP 410 - Humane Intervention Points
ÌýSOP 402 - Tissue Collection for Genotyping - Mice	ÌýSOP 412 - Humane Intervention Points for Aging Rodents
ÌýSOP 403 - Guidelines Blood Collection Volumes and Frequency	ÌýSOP 413 - Tissue Collection for Genotyping - Rats
SOP 404 - Substance Administration	SOP 414 - Rodent Algesiometric Testing
SOP 405 - Monoclonal Antibody Production - Mice	ÌýSOP 415 - Humane Intervention Points for Rodent Cancer Models
SOP 406 - Polyclonal Antibody Production - Rabbits	SOP 417 - Food and Water Restriction in Rodents
SOP 407 - Polyclonal Antibody Production - Rats	SOP 418 - Humane Intervention Points for Animals Used in Training
SOP 409 - Maintaining Clinical Records	SOP 419 - Humane Intervention Points for Rodents Models Involving Paralysis
Ìý	SOP 420 - Estrous Cycle Assessment in Rodents
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This Monitoring Log is to be prepared by the Facility Animal Care Committee as per the approved Animal Use Protocol: ÌýMonitoring log TEMPLATE Ìý
ÌýCMARC NHP Activity Tracking and Procedures Requiring Anesthesia Log

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ÌýSOP 501- Animal Transport and Use Outside of Animal Facilities	ÌýSOP 522 - Environmental Enrichment Program for Fish
ÌýSOP 502 - Environmental Enrichment Program for Rodents	ÌýSOP 523 -ÌýEnvironmental Enrichment Program for Amphibians
ÌýSOP 504 - Environmental Enrichment Program for Rabbits	ÌýSOP 525 - Cat Husbandry
ÌýSOP 505 - Environmental Enrichment Program for Hamsters	ÌýSOP 526 - Environmental Enrichment Program for Cats
ÌýSOP 506 - Environmental Enrichment Program for Guinea Pigs	ÌýSOP 527 - Finch Husbandry
ÌýSOP 507 - Environmental Enrichment Program for NHPs	ÌýSOP 528 - Environmental Enrichment Program for Birds
ÌýSOP 508 - Rodent Husbandry	ÌýSOP 531 - Animal Acquisition Receiving and Acclimation
SOP 509 - Rodent Handling and Restraint	SOP 532 - Vermin Control Program
ÌýSOP 513 - Marmoset Husbandry	SOP 533 - Microbiological Monitoring Program
ÌýSOP 515 - Marmoset Feeding	SOP 536 - Procedure Room Maintenance
ÌýSOP 516Ìý- Marmoset Cage Changing	SOP 537 - Working in Laminar Flow Hood or Animal Transfer Station or Biological Safety Cabinet
ÌýSOP 517 - Marmoset Room Sanitation	SOP 540 - Operating Room Maintenance
ÌýSOP 518 - Transportation of NHPs Between Facilities	SOP 541 - Vendor Approval for Animal Feed and Bedding Suppliers
ÌýSOP 519 - Aquatic Animal Husbandry	Ìý
ÌýSOP 521 - Macaque Husbandry	Ìý

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ÌýSOP 601 - Veterinary Care Program	ÌýSOP 614 - Guidelines on Wire-Bottom Caging for Rodents
ÌýSOP 602 - Rodent Health Monitoring Program	ÌýSOP 615 - Rodent Irradiation
ÌýSOP 603 - Rodent Quarantine Program	ÌýSOP 616 - Rodent Identification
ÌýSOP 604 - Bioexclusion Levels for Rodents	ÌýSOP 619 - Common Rodent Treatment
ÌýSOP 605 - Rodent Import	ÌýSOP 622 - Marmoset Health Monitoring Program
ÌýSOP 606 - Reporting Concerns Regarding Animal Care and Use	ÌýSOP 623 - Marmoset Breeding Colony Management
ÌýSOP 607 - Long-term Housing	ÌýSOP 626 - Macaque Health Monitoring Program
ÌýSOP 608 - Mouse Breeding Colony Management	ÌýSOP 631 - Macaque Herpes B Testing
ÌýSOP 609 - Rat Breeding Colony Management	ÌýSOP 632 - Animal Welfare Assessment
ÌýSOP 610 - Testing of Biological Material	ÌýSOP 636 - Storage and Transport of Primate Tissues
ÌýSOP 611 - Excluded Pathogens - Rodent Facilities	ÌýSOP 637 - Cumulative Endpoints
ÌýSOP 612 - Working with Immunodeficient Mice	ÌýSOP 638 - Positive Reinforcement Training
ÌýSOP 613 - Working with Rodents in the Imaging Facility	Ìý

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ÌýSOP 701 - Macaque-Related Injury	ÌýSOP 709 - Waste Anesthetic Gas Scavenging
ÌýSOP 702 - Animal Related Injury	ÌýSOP 710 - Personal Protective Equipment Requirements
ÌýSOP 703 - Use of Viral Vectors in Neuroscience - Rodents	ÌýSOP 715 - Use of Cisplatin in Rodents
ÌýSOP 705 - Animal Containment Level 2 – Rodents	ÌýSOP 716 - Use of Doxorubicin in Rodents
ÌýSOP 706 - Use of Hazardous Agents in Rodents - General	ÌýSOP 717- Use of Tamoxifen in Rodents
ÌýSOP 708 - Use of Streptozotocin in Rodents	Ìý

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ÌýDC-101ÌýBio Security Cattle Complex	DC-606 Post-Milking Procedure
ÌýDC-121 Flushing Gutters in the Dairy Barn	DC-607 Milking Procedure
ÌýDC-131 Environmental Monitoring and Maintenance	DC-608 Milking Fresh Cows
DC-142 Infectious Agents and Zoonoses	DC-609 Milking Pail Cows
ÌýDC-206 Box Stall Sanitation	DC-610 Milking Cows with Box Stall Milker
ÌýDC-207 Tie Stall Sanitation	DC-611 Milking Staphylococcus Aureus Positive Cows
ÌýDC-208 Heifer Pen_Stall Sanitation	DC-612 Milking Antibiotic Treated Cows
ÌýDC-209 Calf Pen Sanitation	DC-613 Accidental Milking Antibiotic Treated Cows into Milking System
ÌýDC-210 Acquisition of Dairy Cattle	DC-614 Dry Treating Cows
ÌýDC-211 Loading and Transporting Cattle	DC-615 Milk Sampling
ÌýDC-212 Handling and Moving Cattle	DC-616 Milk Culturing
ÌýDC-215 Selecting Appropriate Stalls for Tethered Cattle	DC-617 California Mastitis Test (CMT)
ÌýDC-230 Environmental Enrichment Program for Dairy Cattle	DC-701 Substance Administration for Dairy Cattle
ÌýDC-301 Body Condition Scoring for Dairy Cows	DC-702 Blood Sample Collection
ÌýDC-302 Lameness Scoring for Dairy Cows	DC-712 Pre- and Post-Operative Procedures and Long-Term Care of Fistulated Cows
ÌýDC-303 Injury Scoring for Dairy Cows	DC-713 Rumen Content Collection from Fistulated Cow
ÌýDC-304 Cleanliness Assessment for Dairy Cows	DC-714 Transfaunation
ÌýDC-305 Mastitis Monitoring and Detection	DC-715 Controlled Internal Drug Release Silicone Device Insertion
ÌýDC-306 Mastitis Treatment	DC-A-1A Environmental Monitoring Log
ÌýDC-307 Extra Teat Removal Calves	DC-A-2A Cattle Transport Decision Tree
ÌýDC-311 Managing Downed Cattle	DC-A-3A Disbudding Dehorning Weight and Medication Charts
DC-312 Monitoring, Identification and Treatment of Dairy Cow Health	DC-A-3C Trainer Use Record
DC-313ÌýMonitoring, Identification, and Treatment of Calf Health	DC-A-4A Heat Cycle, Signs and Conception rates of Dairy Cattle
ÌýDC-315 Cumulative Endpoints Dairy Cattle	DC-A-4B Signs of Heat in Dairy Cattle
DC-320ÌýManaging Dirty Cows	DC-A-4C Factors Influencing Heat Detection in Dairy Cattle
ÌýDC-360 Outdoor Exercise Habituation of Tie-Stall Cattle	DC-A-4D Leg Band Color Legend
ÌýDC-361 Outdoor Exercise of Tie-Stall Dairy Cattle	DC-A-5A Colostrum Quality Readings
ÌýDC-400 Heat Detection and Breeding	DC-A-5B Newborn Heifer Feeding Log
DC-405 Pre-Partum Preparation of Dairy Cattle	DC-A-5C Daily Calf Monitoring Log
DC-406 Post-Partum Care of Dairy Cattle	DC-A-6A Herd Health Scoring Record
DC-407 Calving Cows-Heifers	DC-A-6B Injury Scoring Record for Dairy Cows
DC-501 Colostrum Management	DC-A-6C Lameness Scoring Record for Dairy Cows
DC-502 Milk Replacer Preparation	DC-A-6D Cleanliness Assessment Record for Dairy Cows
DC-503 Calf Identification	DC-A-6E Assessment of Rumen Fill
DC-504 Newborn Calf Care	Ìý
DC-505 Calf Care Feeding	Ìý
DC-506 Tube Feeding a Calf	Ìý
DC-601 Milk House Pre-Milking Set Up	Ìý
DC-602 Milk House Post-Milk Wash	Ìý
DC-603 Bulk Tank Sanitizing Procedure	Ìý
DC-604 Box Stall Milker Sanitization	Ìý
DC-605 Preparing Cows for Milking	Ìý

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PU-101 Biosecurity Poultry Unit	PU-501 Handling Restraint
ÌýPU-202 Environmental Management Broiler	ÌýPU-520 Poultry Euthanasia
ÌýPU-203 Broiler Barn Preparation	ÌýPU-A-0 Donald McQueen Shaver Poultry Unit Floor Plan
ÌýPU-204 Sanitation Procedure Broiler Barn	ÌýPU-A-2A Broiler Temperature Management Chart
ÌýPU-205 Manure Management Broiler	ÌýPU-A-3A Should this bird be loaded - Decision tree
ÌýPU-208 Loading and Transporting Broilers	ÌýPU-A-3B Handling Guidelines for Catching Crews
ÌýPU-209 Receiving Broiler Chicks and Poults	Ìý
PU-2010 Environmental Enrichment Program for Poultry	Ìý

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ÌýSW-2010 Environmental Enrichment Program for Swine

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